Including learning experience
2007.08-2009.07 Shanghai Jiao Tong University, Post-doctor, Supervisor: Zeguang Han.
2004.09-2007.06 Fudan University, Analytical Chemistry, PhD, Supervisor: Pengyuan Yang.
2000.09-2003.06 Xuzhou Medical College, Oncology, Master, Supervisor: Xu Wang.
1993.09-1997.06 Xuzhou Medical College, Clinical medicine, Bachelor.
1984.09-1987.06 Jiangsu Health School, Medical laboratory.

 

Work Experience
2015.01-present, Shanghai Jiao Tong University, Institute of Systems Biomedical Sciences, Researcher.
2011.06-2015.01, Shanghai Chip Center, Tumor Translational Medicine Laboratory, Researcher.
2009.11-2011.05, BGI, Health Division, Researcher.
2004.07-2009.10, National Human Genome Southern Research Center, Functional Genomics, Researcher.
2000.02-2004.06, National Human Genome Southern Research Center, Functional Genomics, Visiting researcher.
1997.07- 2000.01, Xuzhou Medical Affiliated Hospital, Central laboratory, Chief laboratory technician.

 

Talent Awards
1. Jian Huang (1/1), National Health Commission, China Society of Health Information and Healthcare Big Data, Intelligent Health Medical Innovation Application Practice Social Influence Excellence Award. The intelligent medical screening informatization in two types of cancer and the big data system to help the long-term mechanism construction of cancer prevention and control in Chinese community. 2020.
2. Jian Huang (2/5), The First Prize of Natural Science of the Ministry of Education. 2017.
3. Jian Huang (4/6), Shanghai Medical Science and Technology Award, Promotion Award. 2016.
4. Jian Huang (1/6), Shenzhen Natural Science Award, The First Prize. 2015.
5. Jian Huang (1/1), Chinese Medical Science and Technology Award, The First prize. Basic and clinical progress of liver disease related to hepatitis mutual infection. 2013.
6. Jian Huang, Selected as Shanghai Excellent Technical Leader, Talent Program. 2012.
7. Jian Huang (1/1), The 6th Excellent Scientific and Technological Paper of Shanghai Pudong New District, The First Prize. 2011.
8. Jian Huang (1/1), Meiji Dairy Life Science Award. 2008.
9. Jian Huang (1/1), Shanghai Natural Science Award. The Second Prize. Functional genomics Research of hepatitis B virus-related liver cancer.2007.

 

Social Service
1. Member of the Gynecological Oncology Committee, Chinese research hospital.
2. Member of the Dermatology and Venereology Group, Society of Tropical and Parasitic Diseases, Chinese Medical Association.
3. Member of the Group of Experimental Animal Immunology and Cell Therapy, Chinese Medical Association.
4. Member of the Standing Committee of HPV Infection Diseases, Precision Medicine Association across the Taiwan Straits.
5. Member of the Standing Committee of Immunology and Cell Therapy Group, Chinese Research Hospital.

1. Basic systematic biomedical research in cancers: Using NGS，gene microarray and bioinformatics technologies, the variances of gene expression, methylation/(hydroxy)methylation as well as the variants in the immune repertoire in cervical or hepatocellular carcinoma were systematically analyzed. And these variants were explored the relevance of tumor clinical features. Combined cytological and zoological techniques were used to identify the tumor-associated genes. The molecular mechanisms of these genes in the development of tumors were investigated. These results will provide new indicators for clinical diagnosis of tumors, or potential drug targets and available drugs for clinical treatment of tumors.
2. Research and development of intelligent screening technology for cervical cancer screening: The development and application of the cervical cancer screening smart medical management system in China's community cervical cancer screening, greatly improving the detection rate of the abnormal results. The system will provide a solution for imprecise sampling, unmanaged process, unintegrated technology and provide theoretical basis and technical support for the establishment of a standardized system of cervical cancer screening and a long-term mechanism of cervical cancer prevention and treatment system in China.
3. Big data information system construction for women's disease diagnosis, treatment and prevention: establish the first big data platform for the prevention and control of cervical cancer in Chinese communities (currently 200,000 online users). Participated in 5G Health Strategy Pilot Project launched by All-China Women's Federation (Carnation Care Initiative) and the National Health Commission/Ministry of Industry and Information Technology of China. Participated in Real-World Study And Health Economics Evaluation Of Cervical Cancer Screening Project by National Center for Women's Health, China CDC. Nearly 200 primary care institutions in Kashgar (Xinjiang), Minhang Dist. (Shanghai), Lishui (Zhejiang), Zhenjiang (Jiangsu) and Nanjing (Jiangsu) cooperated in the prevention and control of women's diseases including cervical cancer, and have achieved great results.

More than 50 papers have published in high level journal, such as J Infect, Nature Genet, Genome Biol, Cancer Res, JCI, J Hepatol etc., with more than 3000 citations. Representative papers are as follows.
1. Yiyan Sun, Xiaohuan Xia, Diksha Basnet, Jialin C. Zheng*, Jian Huang*, Jianhui Liu*. Mechanisms of Ferroptosis and Emerging Links to the Pathology of Neurodegenerative Diseases. Front Aging Neurosci. 2022 Jun; 14: 904152.
2. Na An, Jian Huang*. Meta analysis of the HPV detection in cervical cancer screening by self-sampling of female and clinician-sampling. Genom & Applied Biol. 2021; 40(11/12): 3749-3764.
3. Hanyi Tan, Yue Liu, Chaochao Gong, Jiawei Zhang, Jian Huang*, Qian Zhang*. Synthesis and evaluation of FAK inhibitors with a 5-fluoro-7H-pyrrolo[2,3-d] pyrimidine scaffold as anti-hepatocellular carcinoma agents. Eur J Med Chem. 2021 Jun; 223:113670.
4. Chaohui Bao, Hong Xie, Ling Xu, Boping Zhou, Jun Luo, Wanqiu Huang, Jian Huang*. Identifying potential neoantigens for cervical cancer immunotherapy using comprehensive genomic variation profiling of cervical intraepithelial neoplasia and cervical cancer. Front Oncol. 2021 Jun;11:672386.
5. Yingxin Han, Liyan Ji, Yanfang Guan, Yinge Xue, Yinxin Zhang, Pansong Li, Jiayin Wang, Wanqiu Huang, Hong Xie, Ling Xu, Boping Zhou, Jun Luo, Mengya Ma, Xin Yi, Fei Gao*, Jian Huang*. An epigenomic landscape of cervical intraepithelial neoplasia and cervical cancer using single-base resolution methylome and hydroxymethylome. Clin & Trans Med. 2021 Jul;11(7):e498.
6. Wanqiu Huang, Dachuan Lin, Cuini Wang, Chaohui Bao, Zhaoqi Zhang, Xinchun Chen, Zheng Zhang, Jian Huang*. The determination of release from isolation of COVID-19 patients requires ultra-high sensitivity nucleic acid test technology. J Infect. 2021 Jan;82(1):159-198.
7. Haoqiang Wan, Lanlan Ge, Lingyun Xiao, Jiemei Li, Weigang Wu, Shusong Peng, Jian Huang*, Boping Zhou*, Xiaobin Zeng*. 3,4,5-Tri-O-caffeoylquinic acid methyl ester isolated from Lonicera japonica Thunb. Flower buds facilitates hepatitis B virus replication in HepG2.2.15 cells. Food Chem Toxicol. 2020 Apr; 138:111250.
8. Jian Huang, Jianxiang Geng. Expert consensus on integration program for cervical cancer screening and management measures for abnormal population. J Med Post. 2020;33(3): 230-233.
9. Fei Ma, Yanfang Guan, Zongbi Yi, Lianpeng Chang, Qiao Li 1, Shanshan Chen, Wenjie Zhu, Xiuwen Guan, Chunxiao Li, Haili Qian, Xuefeng Xia, Ling Yang, Jianjun Zhang, Hatim Husain, Zhongxing Liao, Andrew Futrea, Jian Huang#, Xin Yi#, Binghe Xu#. Assessing tumor heterogeneity using ctDNA to predict and monitor therapeutic response in metastatic breast cancer. Inter J Cancer. 2020 Mar;146(5):1359-1368.
10. Yan An#, Yanfang Guan#, Yaping Xu#, Yingxin Han#, Chi Wu, Chaohui Bao, Boping Zhou, Haiyan Wang, Mingxia Zhang, Weilong Liu, Lin Qiu, Zeguang Han, Yongsheng Chen, Xuefeng Xia, Jiayin Wang, Zhentian Liu, Wanqiu Huang*, Xin Yi*, Jian Huang*. The diagnostic and prognostic usage of circulating tumor DNA in operable hepatocellular carcinoma. Am J Transl Res. 2019 Nov;11(10):6462-6474.
11. Xin Zou, Wei Cao, Jian Huang*, Jie Hao*, Ze-Guang Han*. Optimal Gene Filtering for Single-Cell data (OGFSC) - a gene filtering algorithm for single-cell RNA-seq data. Bioinformatics. 2019 Aug;35(15):2602-2609.
12. Jian Huang#*, Zhaoyang Qian, Yuhua Gong, Yanzhou Wang, Yanfang Guan, Yingxin Han, Xin Yi, Wanqiu Huang, Liyan Ji, Jiajia Xu, Mengyuan Su, Qing Yuan, Shujian Cui, Chaohui Bao, Weilong Liu, Xi Chen, Ming Zhang, Xiaohuan Gao, Renhua Wu, Yinxin Zhang, Huicheng Xu, Shida Zhu, Hongmei Zhu, Ling Yang, Xun Xu, Pingyu Zhou*, Zhiqing Liang*. Comprehensive genomic variation profiling of cervical intraepithelial neoplasia and cervical cancer identifies potential targets for cervical cancer early warning. J Med Genet. 2019 Mar;56(3):186-194.
13. Qianting Yang, Mingfeng Liao, Wenfei Wan, Mingxia Zhang, Qi Chen, Jiubiao Guo, Bin Pen, Jian Huang, Haiying Liu, Ayano Yahagi, Xingzhi Xu, Katsuhiko Ishihara, Andrea Cooper, Xinchun Chen, Yi Cai. CD157 Confers Host Resistance to Mycobacterium tuberculosis via TLR2-CD157-PKCzeta-Induced Reactive Oxygen Species Production. mBio. 2019 Aug;10(4):e01949-19.
14. Liu Yang, Song Ye, Xinyi Zhao, Liyan Ji, Yinxin Zhang, Pingyu Zhou, Jun Sun, Yanfang Guan, Yingxin Han, Chao Ni, Xiaoge Hu, Weilong Liu, Haiyan Wang, Boping Zhou, and Jian Huang*. Molecular Characterization of HBV DNA Integration in Patients with Hepatitis and Hepatocellular Carcinoma. J Cancer. 2018 Sep;9(18):3225-3235.
15. Qianting Yang, Mingfeng Liao, Wenfei Wang, Mingxia Zhang, Qi Chen, Jiubiao Guo, Bin Peng, Jian Huang, Haiying Liu, Ayano Yahagi, Xingzhi Xu, Katsuhiko Ishihara, Andrea Cooper, Xinchun Chen, Yi Cai. Aberrant Humoral Immune Responses in Neurosyphilis: CXCL13/CXCR5 Play a Pivotal Role for B-Cell Recruitment to the Cerebrospinal Fluid. J Infect Dis. 2017 Sep; 216(5):534-544.
16. Yingxin Han, Hongmei Li, Yanfang Guan, Jian Huang*. Immune repertoire: a potential biomarker and therapeutic for hepatocellular carcinoma. Cancer Lett. 2016 Sep; 379(2):206-212.
17. Xueda Hu, Shengqing Wan, Ying Ou, Boping Zhou, Jialou Zhu, Xin Yi, Yanfang Guan, Wenlong Jia, Xing Liu, Qiudao Wang, Yao Qi, Qing Yuan, Wanqiu Huang, Weijia Liao, Yun Wang, Qinghua Zhang, Huasheng Xiao, Xinchun Chen, Jian Huang*. RNA over-editing of BLCAP contributes to hepatocarcinogenesis identified by whole-genome and transcriptome sequencing. Cancer Lett. 2015 Feb;357(2):510-519.
18. Fei Gao, Yudong Xia, Junwen Wang, Zhilong Lin, Ying Ou, Xing Liu, Weilong Liu, Boping Zhou, Huijuan Luo, Baojin Zhou, Bo Wen, Xiuqing Zhang, Jian Huang*. Integrated analyses of DNA methylation and hydroxymethylation reveal tumor suppressive roles of ECM1, ATF5 and EOMES in human hepatocellular carcinoma. Genome Biol. 2015 Jan;15(12):533.
19. Jian Huang#, Qing Deng#, Qun Wang#, Kunyu Li, Jihong Dai, Niu Li, Zhidong, Bo Zhou, Xiaoyan Liu, Ruifang Liu, Qianlan Fei, Hui Chen, Bing Cai, Boping Zhou, Husheng Xiao, Lunxiu Qin, Zeguang Han. Exome sequencing of hepatitis B virus-associated hepatocellular carcinoma. Nat Genet. 2012 Aug;44(10):1117-1121.
20. Jian Huang#, Dali Zheng#, Yun-Li Zhang, Xiao-Mei Teng, Hui Chen, Ze-Guang Han. Genetic and epigenetic silencing of scavenger receptor SCARA5 as a candidate tumor suppressor could contribute to hepatocellular carcinoma via activation of FAK. J Clin Invest. 2010 Jan;120(1):223-241.
21. Dali Zheng, Li Zhang, Xiao Xu, Qing Deng, Xiaomei Teng, Kesheng Wang, Xin Zhang, Jian Huang, Zeguang Han. Epigenetic modification induced by hepatitis B virus X protein via the relocation of de novo DNA methyltransferase DNMT3A. J Hepatol. 2009 Feb;50(2):377-387.
22. Qing Deng, Qun Wang, Weiying Zong, Da-Li Zheng, Yixin Wen, Kesheng Wang, Xiaomei Teng, Xin Zhang, Jian Huang, Zeguang Han*. E2F8 contributes to human hepatocellular carcinoma via regulating cell proliferation. Cancer Res. 2010 Jan; 70(2):782-791.
23. Stefanska B, Jian Huang, Bhattacharyya B, Suderman M, Hallett M, Zguang Han, Szyf M. Definition of the landscape of promoter DNA hypomethylation in liver cancer. Cancer Res. 2011 Sep; 71(17):5891-5903.
24. Xiang-Ru Xu, Jian Huang, Zhigang Xu, Zhu Chen and Zeguang Han. Insight into hepatocellular carcinogenesis at transcriptome level by comparing gene expression profiles of hepatocellular carcinoma with those of corresponding noncancerous liver. Proc Natl Acad Sci U S A. 2001 Feb; 98 (26): 15089-15094.

1. Key Project of Shanghai Science and Technology Commission: Preclinical study of a novel drug THY-10A62 targeting FAK for liver cancer treatment, 19Z111220022, 2019-2022, 480,000 (Completed, Project leader).
2 .The Interdisciplinary Program of Medicine and Engineering of Shanghai Jiao Tong University: Single-cell omics technology and its translational research in the precision diagnosis and treatment of liver cancer, Department of Research, 19X190020005, 2019-2022, 1 million (In research, Project leader).
3. National Natural Science Foundation of China General Project: The role and mechanism of SLC7A11 interacting protein HSPB1 in the regulation of ferroptosis in liver cancer, 81872274, 2018-2022, 570,000 (In research, Project leader).
4. Special Fund of Shanghai Science and Technology Commission: Design and synthesis of novel inhibitors of hepatocellular carcinoma targeting FAK and their feasibility in the treatment of hepatocellular carcinoma, 15431902900, 2016-2018, 500,000 (Completed, Project leader).
5. National Natural Science Foundation of China: The relationship between RNA editing and the occurrence and development of liver cancer, 81472639, 2014-2017, 750,000 (Completed, Project leader).
6. 973 Major Basic Project of the Ministry of Science and Technology: Component analysis, condition optimization and functional simulation of the microenvironment of inducible neural progenitor cells in the forebrain, 2014CB965002, 2014-2018, 1 million (Completed, Project leader).
7. General Project of National Natural Science Foundation of China: The relationship between DNA hydroxymethylation and gene expression level and clinical characteristics of liver cancer, 81272306, 2012-2015, 700,000 (Completed, Project leader).
8. 863 Key Research Project of the Ministry of Science and Technology: Genome technology of major Diseases, Research on the interaction between cells and pathogen genomes of HBV-related liver cancer, 2012AA02A205, 2012-2015, 4 million (Completed, Sub-project leader).
9. Key Basic Research Project of Shanghai Science and Technology Commission: The relationship between DNA hydroxymethylation and gene expression and clinical characteristics of liver cancer, 11JC1408800, 2012-2014, 200,000 (Completed, Project leader).
10. Shanghai Outstanding Technology Leader Program: Development and application of early diagnosis technology for liver cancer based on peripheral blood gene expression profiling, 12XD1421400, 2012-2014, 400,000 (Completed, Project leader).
11. 973 Project of the Ministry of Science and Technology: Clinical transformation research of leukemia based on systems biomedicine-related theory exploration and research, 2010CB529206, 2012-2014, 800,000 (Completed, Sub-project leader).
12. Shenzhen Biological Industry High Technology Industry Project: Application of new gene detection technology to screen HPV and prevent cervical cancer, Shenzhen 20101324, 2010-2012, 5 million (Completed, Project leader).
13. Guangdong Innovation Research Team Fund: International Cancer Genomics Research, 2010-2014, 30 million (Completed, Sub-project leader).
14. Basic Research Key Project of Shanghai Science and Technology Commission: Functional study of potential tumor suppressor gene SCARA5 in liver cancer, 08JC1416400, 2008-2010, 300,000 (Completed, Project leader).
15. 973 Project: Transcriptome breadth Preview: transcriptome study of human liver cells, gastric mucosal cells and their related cancer cells, 2006CB910402, 2006-2010, 700,000 (Completed, Sub-project leader).
16. Prevention and Treatment of AIDS, viral hepatitis and other Major Infectious Diseases Science and Technology Major Project of Ministry of Health: Screening and validation of molecular markers and intervention targets related to recurrence and metastasis of liver cancer, 2008ZX10002-021, 2008-2010, 2.8 million (Completed, Co-leader).
17. Shanghai Science and Technology Foundation Project of China: Analysis of transcriptome data of human multiple tissues by massively parallel signal tag sequencing technology, 06ZR14069, 2006-2008, 80,000 (Completed, Project leader).
18. Shanghai Science and Technology "Mountaineering Program" Research Project: Human full-length gene cloning resource sharing service construction, 06DZ22903, 2006-2008, 500,000 (Completed, Project leader).
19. 863 Key Research Project: ORF and linkage map of important protein interactions in Chinese human Liver Proteomics Program, 2004BA711A19, 2004-2005, 3.7 million (Completed, Project leader).
20. Postdoctoral Research Project of China (First class support): Association between epigenetic abnormalities and gene expression and clinical features in liver cancer, 2007-2009, 20070420097, 50,000 (Completed, Project leader).
21. Postdoctoral Support Program of Shanghai of Science and Technology Commission: Association between DNA copy number variation and transcriptome abnormalities in hepatocellular carcinoma, 2007-2009, 7R214138, 20,000 (Completed, Project leader).
22. Postdoctoral Support Program of Pudong New District, Shanghai: Discovery and development of diagnostic indicators and drug targets for liver cancer, 2007-2009, 50,000 (Completed, Project leader).

More than 40 patents have been granted/applied in several countries and regions.

Cultivate 3 Doctoral students (graduated), 2 Doctoral students (in progress);
Cultivate 2 Master students (graduated) and 1 Master student (co-training student).