师资队伍

黄健

 

电话: 021-34205108
传真:
邮箱: jianhuang@sjtu.edu.cn
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学习经历
• 1984.9-1987.6,江苏省卫生学校,医学检验;
• 1993.9-1997.6,江苏徐州医学院,临床医学;
• 2000.9-2003.6, 江苏徐州医学院(硕士研究生),肿瘤学;
• 2004.9-2007.6, 复旦大学(博士研究生),分析化学。
• 2007.9-2009.6,上海交通大学(博士后),肿瘤基因组学和分子遗传学

 

工作经历
• 1987.8-1995.6, 徐州医学附属医院检验科,医学检验师;
• 1995.7-1997.6, 徐州医学附属医院医务科,主管检验师;
• 1997.7-2001.1, 徐州医学附属医院中心实验室,主管检验师;
• 2001.2-2004,6 国家人类基因组南方研究中心,客座研究;
• 2004.7-2009.11, 国家人类基因组南方研究中心,课题组长;
• 2009.11-2011.6 深圳华大基因研究院健康事业研发中心主任;
• 2011.7-2015.5生物芯片上海国家工程研究中心肿瘤基因组学转化应用研究室主任;
• 2011.6-至今 国家人类基因组南方研究中心/上海市疾病与健康基因组学省部共建重点实验室课题组长;
• 2015.6-至今 上海交通大学系统生物医学研究院/疾病基因组学研究室主任,系统生物医学教育部重点实验室副主任。

 

人才奖项
上海市优秀技术带头人

 

1. 肿瘤基因组变异的基础和应用研究
2. 肝癌发病机制研究及治疗药物研发
3. 宫颈癌筛查、早诊和预后技术研究

1. Jian Huang#*, Zhaoyang Qian, Yuhua Gong, Yanzhou Wang3, Yanfang Guan, Yingxin Han, Xin Yi, Wanqiu Huang, Liyan Ji, Jiajia Xu, Mengyuan Su, Qing Yuan, Shujian Cui, Chaohui Bao, Weilong Liu, Xi Chen, Ming Zhang, Xiaohuan Gao, Renhua Wu, Yinxin Zhang, Huicheng Xu, Shida Zhu, Hongmei Zhu, Ling Yang, Xun Xu, Pingyu Zhou*, Zhiqing Liang*. Comprehensive genomic variation profiling of cervical intraepithelial neoplasia and cervical cancer identifies potential targets for cervical cancer early warning. Journal of Medical Genetics. 2018 Dec 19. pii: jmedgenet-2018-105745. doi: 10.1136/jmedgenet-2018-105745.
2. Yan An†, Yanfang Guan†, Yaping Xu†, Yingxin Han†, Chi Wu4, Chaohui Bao, Boping Zhou, Haiyan Wang, Mingxia Zhang, Weilong Liu, Lin Qiu1, Zeguang Han, Yongsheng Chen, Xuefeng Xia, Jiayin Wang, Wanqiu Huang*, Xin Yi*, Jian Huang*. Blood circulating DNA content and mutations for diagnosis and prognosis determination of hepatocellular carcinoma using targeted panel sequencing. Molecular Oncology, 2019, submitted.
3. Xin Zou, Wei Cao, Jian Huang*, Jie Hao*, Ze-Guang Han*. Optimal Gene Filtering for Single-Cell data (OGFSC) – a gene filtering algorithm for single-cell RNA-seq data. Bioinformatics. 2018 Dec 10. doi: 10.1093/bioinformatics/bty1016.
4. Liu Yang, Song Ye, Xinyi Zhao, Liyan Ji, Yinxin Zhang, Pingyu Zhou, Jun Sun, Yanfang Guan, Yingxin Han, Chao Ni, Xiaoge Hu, Weilong Liu, Haiyan Wang, Boping Zhou, and Jian Huang*. Molecular Characterization of HBV DNA Integration in Patients with Hepatitis and Hepatocellular Carcinoma. Journal of Cancer. J Cancer. 2018 Sep 7;9(18):3225-3235. doi: 10.7150/jca.26052. eCollection 2018.
5. Yu Q, Cheng Y, Wang Y, Wang C, Lu H, Guan Z, Huang J, Gong W, Shi M, Ni L, Wu J, Peng R, Zhou P. Aberrant Humoral Immune Responses in Neurosyphilis: CXCL13/CXCR5 Play a Pivotal Role for B-Cell Recruitment to the Cerebrospinal Fluid. J Infect Dis. 2017 Sep 1; 216(5):534-544.
6. Yu YQ, Li J, Liao Y, Chen Q, Liao WJ, Huang J*. The preoperative alkaline phosphatase-to-platelet ratio index is an independent prognostic factor for hepatocellular carcinoma after hepatic resection. Medicine (Baltimore). 2016 Dec; 95(51):e5734.
7. Qiudao Wang, Yan An. Ying Ou, Qing Yuan, Yao Qi, Junhui Chen, Jian Huang*. Identification of allelic expression imbalance genes in human hepatocellular carcinoma through massively parallel DNA and RNA sequencing. Med Oncol. 2016 Apr; 33(4):38. doi: 10.1007/s12032-016-0751-y. Epub 2016 Mar 21.
8. Jun Sun, Zhefeng Meng, Kaiqi Wu, Yuezhu Wang, Huajun Zheng, Jian Huang*, Pingyu Zhou*. Tracing the origin of Treponema pallidum in China using next generation sequencing. Oncotarget. 2016 Jul 12; 7(28):42904-42918. doi: 10.18632/oncotarget.10154.
9. Xing Liu, Weijia Liao, Qing Yuan, Ying Ou, Jian Huang*. TTK can significantly promote the proliferation and migration of hepatocellular carcinoma cells as a novel therapeutic target via Akt activation. Oncotarget. 2015 Oct 27;6(33):34309-20. doi: 10.18632/oncotarget.5295.
10. Changhai Tian, Yuju Li, Yunlong Huang, Yongxiang Wang, Dapeng Chen, Jinxu Liu, Xiaobei Deng, Lijun Sun, Kristi Anderson, Xinrui Qi, Yulong Li, R. Lee Mosley, Xiangmei Chen, Jian Huang, Jialin C. Zheng. Selective Generation of Dopaminergic Precursors from Mouse Fibroblasts by Direct Lineage Conversion. Sci Rep. 2015 Jul 30; 5: 12622. doi: 10.1038/srep12622.
11. Yingxin Han, Hongmei Li, Yanfang Guan, Jian Huang*. Immune repertoire: a potential biomarker and therapeutic for hepatocellular carcinoma. Cancer Lett. 2015 Jul 15. pii: S0304-3835(15)00441-3. doi: 10.1016/j.canlet.2015.06.022.
12. Weijia Liao, Weilong Liu, Xing Liu, Qing Yuan, Ying Ou, Yao Qi, Wanqiu Huang, Yun Wang, Jian Huang*. Upregulation of FAM83D affects the proliferation and invasion of hepatocellular carcinoma. Oncotarget. 2015 Sep 15;6(27):24132-47.
13. Yingxin Han, Xing Liu, Yuqi Wang, Xiaolei Wu, Yanfang Guan, Xinchun Chen, Boping Zhou, Qing Yuan, Ying Ou, Renhua Wu, Wanqiu Huang, Yun wang, Ming Zhang, Yinxin Zhang, Dongxing Zhu, Hongmei Zhu, Ling Yang, Xin Yi, Chen Huang & Jian Huang*. Identification of characteristic TRB V usage in HBV-associated HCC by using differential expression profiling analysis. Oncoimmunology. 2015 Apr 2;4(8):e1021537. eCollection 2015.
14. Zhang ZZ, Huang J, Wang YP, Cai B, Han ZG. NOXIN as a cofactor of DNA polymerase-primase complex could promote hepatocellular carcinoma. Int J Cancer. 2015 Aug 15;137(4):765-75.
15. Hu X, Wan S, Ou Y, Zhou B, Zhu J, Yi X, Guan Y, Jia W, Liu X, Wang Q, Qi Y, Yuan Q, Huang W, Liao W, Wang Y, Zhang Q, Xiao H, Chen X, Huang J*. RNA over-editing of BLCAP contributes to hepatocarcinogenesis identified by whole-genome and transcriptome sequencing. Cancer Lett. 2015 Feb 28;357(2):510-9.
16. Gao F, Xia Y, Wang J, Lin Z, Ou Y, Liu X, Liu W, Zhou B, Luo H, Zhou B, Wen B, Zhang X, Huang J*. Integrated analyses of DNA methylation and hydroxymethylation reveal tumor suppressive roles of ECM1, ATF5 and EOMES in human hepatocellular carcinoma. Genome Biol. 2015 Jan 3;15(12):533.
17. Cai Y, Yang Q, Tang Y, Zhang M, Liu H, Zhang G, Deng Q, Huang J, Gao Z, Zhou B, Feng CG, Chen X. Increased complement C1q level marks active disease in human tuberculosis. PLoS One. 2014 Mar 19;9(3):e92340.
18. Ying Gu, Kangmo Lu, Guanghui Yang, Zhong Cen, Li Yu, Lin Lin, Jing Hao, Zhigang Yang, Jiabao Peng, Shujian Cui, Jian Huang*. Mutation spectrum of six genes in Chinese phenylketonuria patients obtained through next-generation sequencing. PLoS One. 2014; 9(4):e94100.
19. Weijia Liao, Weilong Liu, Qing Yuan, Xing Liu, Ying Ou, Songqing He, Shengguang Yuan, Liling Qin, Qian Chen, Kate Nong, Minghui Mei, Jian Huang*. Silencing of DLGAP5 by siRNA significantly inhibits the proliferation and invasion of hepatocellular carcinoma cells. PLoS One. 2013; 8(12):e80789.
20. Wei X, Ju X, Yi X, Zhu Q, Qu N, Liu T, Chen Y, Jiang H, Yang G, Zhen R, Lan Z, Qi M, Wang J, Yang Y, Chu Y, Li X, Guan Y, Huang J*. Identification of Sequence Variants in Genetic Disease Genes using Targeted Next-Generation Sequencing. PLoS One. 2011;6(12):e29500. Epub 2011 Dec 21.
21. Yao Yu, Jie Ping,Hui Chen, Longxian Jiao, Siyuan Zheng, Ze-Guang Han,Pei Hao,Jian Huang*. A comparative analysis of liver transcriptome suggests divergent liver function among human, mouse and rat. Genomics. 2010 Nov;96(5):281-289.
22. Jian Huang*. Current Progress in Epigenetic Research for Hepatocarcinomagenesis. Sci China C Life Sci. 2009 Jan;52(1):31-42.
23. Jian Huang*, Yun Lin, Lihua Li, Deng Qing, Xiao-Mei Teng, Yun-Li Zhang, Yuanjie Hu, Haihui Sheng, Pengyuan Yang, Ze-guang Han. ARHI, as a novel suppressor of cell growth and down-regulated in human hepatocellular carcinoma, could contribute to hepatocarcinogenesis. Molecular Carcinoma, 2009 Feb;48(2):130-40.
24. Huang J, Deng Q, Wang Q, Li KY, Dai JH, Li N, Zhu ZD, Zhou B, Liu XY, Liu RF, Fei QL, Chen H, Cai B, Zhou B, Xiao HS, Qin LX, Han ZG. Exome sequencing of hepatitis B virus-associated hepatocellular carcinoma. Nature Genetics. 2012 Aug 26;44(10):1117-1121.
25. Jian Huang, Dali Zheng, Yun-Li Zhang, Xiaomei Teng, Hui Chen, Ze-Guang Han. Genetic and epigenetic silencing of scavenger receptor SCARA5 as a candidate tumor suppressor could contribute to hepatocellular carcinoma via activation of FAK. J Clin Invest. 2010 Jan;120(1):223-41.
26. Huang J, Zhang X, Zhang M, Zhu JD, Zhang YL, Lin Y, Wang KS, Qi XF, Zhang Q, Liu GZ, Yu J, Cui Y, Yang PY, Wang ZQ, Han ZG. Up-regulation of DLK1 gene could contribute to human hepatocellular carcinoma. Carcinogenesis. 2007,28(5):1094–1103.
27. Jian Huang, Pei Hao, Hui Chen, Wei Hu, Feng Liu & Ze-Guang Han. Genome-wide identification of Schistosoma japonicum microRNAs using a deep-sequencing approach. PLoS One. 2009 Dec 8;4(12):e8206.
28. Jian Huang, Pei Hao, Yun-li Zhang,Fu-Xing Deng, Qing Deng, Yi Hong, Xiao-Wo Wang, Yun Wang, Peng-Yuan Yang, Xue-Gong Zhang, Yi-Xue Li, Hong-Yang Wang, Ze-Guang Han. Discovering multiplicate transcripts of human hepatocytes using massively parallel signature sequencing (MPSS). BMC Genomics 2007, 8:207 (2 July 2007).
29. Jian Huang, Yun-Li Zhang, Yun Lin, Da-Li Zheng, Ze-Guang Han. Down-regulation of SFRP1 as a tumor suppressor gene can contribute to human hepatocellular carcinoma. BMC Cancer 2007, 7:126.
30. Huang J, Sheng HH, Shen T, Hu YJ, Xiao HS, Zhang Q, Zhang QH, Han ZG. Correlation between genomic DNA copy number alterations and transcriptional expression in hepatitis B virus-associated hepatocellular carcinoma. FEBS Letters. 2006 Jun 26; 580(15):3571-3581.
31. Tingting Li; Jian Huang; Ying Jiang; Yan Zeng; Fuchu He, MIchael Zhang, Zeguang Han, Xuegong Zhang. Multi-stage analysis of gene expression and transcription regulation in C57/B6 mouse liver development. Genomics 93 (2009) 235–242.
32. Stefanska B, Bouzelmat A, Huang J, Suderman M, Hallett M, Han ZG, Al-Mahtab M, Akbar SM, Khan WA, Raqib R, Szyf M. Discovery and validation of DNA hypomethylation biomarkers for liver cancer using HRM-specific probes. PLoS One. 2013; 8(8):e68439.
33. Liu RF, Xu X, Huang J, Fei QL, Chen F, Li YD, Han ZG. Down-regulation of miR-517a and miR-517c promotes proliferation of hepatocellular carcinoma cells via targeting Pyk2. Cancer Lett. 2013 Feb 28;329(2):164-173.
34. Da-Li Zheng, Li Zhang, Xiao Xu, Qing Deng, Xiao-Mei Teng, Ke-Sheng Wang, Xin Zhang, Jian Huang, Ze-Guang Han. Epigenetic modification induced by hepatitis B virus X protein via the relocation of de novo DNA methyltransferase DNMT3A. J Hepatol. 2009 Feb;50(2):377-87.
35. Deng Q, Wang Q, Zong WY, Zheng DL, Wen YX, Wang KS, Teng XM, Zhang X, Huang J, Han ZG. E2F8 contributes to human hepatocellular carcinoma via regulating cell proliferation. Cancer Res. 2010 Jan 15; 70(2):782-91.
36. Stefanska B, Huang J, Bhattacharyya B, Suderman M, Hallett M, Han ZG, Szyf M. Definition of the landscape of promoter DNA hypomethylation in liver cancer. Cancer Res. 2011 Sep 1; 71(17):5891-903.
37. Jiang Y, Ying W … Yang Y, Wang H, Huang J, Han ZG, He Q, Chen P, Liang S, Zhao M, Mao X, Wei L, Yu H, Cao Z, Li Y, Dai W, Jiang H, Wang D, Zheng J, Xue G, Tang Y, Li X, Cheng J, Liu Y, Zhang X, Wang X, Jia J, An D, Wang Z, Li Q, Cui T. First insight into human liver proteome from PROTEOMESKY-LIVERHu 1.0, a publicly-available database. J Proteome Res. 2010 Jan; 9(1):79-94.
38. Fu Y, Huang J, Wang KS, Zhang X, Han ZG. RNA interference targeting CITRON can significantly inhibit the proliferation of hepatocellular carcinoma cells. Mol Biol Rep. 2011 Feb;38(2):693-702.
39. Li T, Wan B, Huang J, Zhang X. Comparison of gene expression in hepatocellular carcinoma, liver development, and liver regeneration. Mol Genet Genomics. 2010 May; 283(5):485-492.
40. Xin Yi, Jingjing Li, Shuang Yu, Ai Peng Zhang,Jiajia Xu, Ji Yi,JingZou, Xifang Nie, Jian Huang, Jian Wang. New MALDI-TOF Mass Spectrometry Method Provides High-Sensitivity, High-Throughout HPV Genotyping. American Journal of Clinical Pathology. 2011 Dec;136(6):913-9.
41. Xu X, Liu RF, Wan BB, Xing WM, Huang J, Han ZG. Expression of a novel gene FAM43B repressing cell proliferation is regulated by DNA methylation in hepatocellular carcinoma cell lines. Mol Cell Biochem. 2011, Apr2.354 (1-2), 11-20.
42. Chen C, Huang C, Chen S, Liang J, Lin W, Ke G, Zhang H, Wang B, Huang J, Han Z, Ma L, Hui K, Yang X, Yang P, He F and Tao T. subunit-subinit interactions in the human 26s proteasome. Proteomics, 2008 Jan 9; 8(3):508-520.
43. Guo Y, Zhang X, Huang J, Zeng Y, Liu W, Geng C, Li KW, Yang D, Wu S, Wei H, Han Z, Qian X, Jiang Y, He F. Relationships between hematopoiesis and hepatogenesis in the midtrimester fetal liver characterized by dynamic transcriptomic and proteomic profiles. PLoS One. 2009 Oct 28;4(10):e7641.
44. Xiang-Ru Xu, Jian Huang, Zhi-Gang Xu … Zhu Chen and Ze-Guang Han. Insight into hepatocellular carcinogenesis at transcriptome level by comparing gene expression profiles of hepatocellular carcinoma with those of corresponding noncancerous liver. Proc. Natl. Acad. Sci. USA. 2001, 98 (26): 15089-15094.

参与发表的专著
1、 疾病相关基因的筛选与克隆,《生物学前沿技术在医学研究中的应用》(第二章)复旦大学出版社,2007,1-30.
2、 肝癌表观遗传学《肿瘤表观遗传学》(第十八章)科学出版社,2011,363-381.
3、 DNA甲基化与肿瘤的精准诊 《精准医学系列从书》(第四章)上海交通大学出版社,2018.3

1. 国家自然基金面上项目:SLC7A11互作蛋白HSPB1在肝癌铁死亡调控中的作用及机制研究. 81872274,2018-2022年,直接费用57万。(在研中,课题负责人)
2. 2015年市科委专项经费, 以肝癌高表达基因FAK为靶点设计、合成肝癌新型抑制剂及其在肝癌治疗中的可行性研究,15431902900,2016-2018年,50万。(在研中,课题负责人)
3. 国家自然基金面上项目:RNA过编辑现象与肝癌发生发展的关系研究,81472639,2014-2017年,75万。(在研中,课题负责人)
4. 科技部973重大基础项目: 诱导性前脑神经祖细胞微环境的组分解析、条件优化和功能模拟,2014CB965002,2014-2018年,100万。(在研中,课题负责人)
5. 国家自然基金面上项目:DNA羟甲基化与肝癌基因表达水平与临床特征的关系研究,81272306,2012-2015年,70万。(已完成,课题负责人)
6. 科技部863重大攻关项目: “重大疾病的基因组技术”的课题“HBV相关肝癌的细胞与病原基因组互作研究”(课题编号2012AA02A205, 2012年1月-2015年12年, 400万)(已完成,子课题负责人)
7. 上海市科委基础研究重点项目基金: DNA羟甲基化与肝癌基因表达及临床特征的关联性研究.(项目编号:11JC1408800 2012年1月-2014年12年, 20万)(已完成,课题负责人);
8. 上海市优秀技术带头人计划项目,基于外周血基因表达谱的肝癌早期诊断技术研发和应用,(项目编号:12XD1421400) (2012.7.1-2014.6.30,40万)(已完成,课题负责人)
9. 973项目,基于系统生物医学基础的白血病临床转化研究-系统生物医学相关理论探索研究(项目编号:2010CB529206。2012年1-2014年12月, 80万)(已完成,子课题负责人);
10. 深圳市生物产业高技术产业项目:应用新型基因检测技术筛查HPV预防宫颈癌,(2010-2012.12,深发改20101324号, 500万)(已完成,项目负责人)
11. 广东省创新研究团队基金:“国际肿瘤基因组学研究” (2010年3月-2014年12月,3000万)(已完成,子课题负责人)
12. 上海市科委基础研究重点项目基金: 肝癌潜在抑癌基因SCARA5的功能研究.(项目编号:08JC1416400 2008年10月-2010年9月,30万)(已完成,负责人)
13. 转录组广度预览:人类肝细胞、胃粘膜细胞及其相关癌细胞的转录组研究(973项目: 2006CB910402,2006年1-2010年12月,70万)((已完成,子课题负责人)
14. 卫生部“艾滋病和病毒性肝炎等重大传染病防治”科技重大专项:肝癌复发转移相关分子标志物和干预靶点筛选和验证: 2008ZX10002-021, 2008-2010,280万((已完成,共同负责人)
15. 上海市科学技术自然基金项目:大规模平行信号标签测序技术检测人类多种组织转录组数据分析。(项目编号:06ZR14069,2006年9-2008年9月,8万)(已完成,负责人)
16. 上海市科学技术“登山计划”科研项目:人类全长基因克隆资源共享服务建设(项目编号:06DZ22903,2006年11-2008年9月,50万)(已完成,负责人)
17. 中国人类肝脏蛋白质组学计划中ORF组及重要蛋白质相互作用连锁图的研究工作 (863重大攻关项目,2004BA711A19,2004年10-2005年12,370万) (已完成,负责人)
18. 中国博士后资助计划(一等资助):肝癌表观遗传学异常与基因表达及临床特征的关联性研究,2007年-2009年,编号:20070420097,项目经费5万(已完成,课题负责人)
19. 上海市博士后资助计划(上海市科委):肝癌DNA拷贝数变异和转录组异常的关联研究,2007年-2009年,编号:7R214138,项目经费2万(已完成,课题负责人)
20. 上海浦东新区博士后资助计划:肝癌诊断指标及药物靶点的发现与开发,2007年-2009年。项目经费5万(已完成,课题负责人)

1. 2017年度教育部自然科学一等奖(第二完成人)
2. 2016年度上海医学科技奖推广奖(第四完成人)
3. 2015年度深圳市自然科学一等奖(第一完成人)
4. 2013年华夏医学科技奖一等奖(肝炎病互感染相关肝病疾病进展的基础和临床)
5. 2012年度入选上海优秀技术带头人 (人才计划)
6. 2011年度上海浦东新区第六届优秀科技论文一等奖(第一完成人)
7. 2008年度明治乳业生命科学奖(科学奖)(第一完成人)
8. 2007年度上海市自然科学二等奖(乙型肝炎病毒相关肝癌的功能基因组学研究)(第二完成人)

在读博士:3人
在读硕士:2人

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